Comparison of Three Commercial Test Systems for Biotyping Haemophilus influenzae and Haemophilus parainfluenzae

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Comparison of three commercial test systems for biotyping Haemophilus influenzae and Haemophilus parainfluenzae.

The biotypes of Haemophilus influenzae and Haemophilus parainfluenzae isolates were determined with three commercially available biochemical test kits: the IDS RapID NH system, the Neisseria-Haemophilus identification test (NHI card), and the API NH strip. The API NH strip performed best, correctly classifying the biotypes of 371 of 380 (97.6%) different challenge strains.

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Rapid Biotyping of Haemophilus influenzae and Haemophilus parainfluenzae with PathoTec Strips and Spot Biochemical Tests BILLIE

PathoTec strips and spot biochemical tests were evaluated for the ability to biotype Haemophilus influenzae and Haemophilus parainfluenzae. Indole, urease, and ornithine decarboxylase reactions were tested. The results of PathoTec strips compared favorably with those conventional methods; the percent agreements were as follows: indole, 100; urease, 99.5; and ornithine, 97.5. Spot tests were sim...

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Rapid biotyping of Haemophilus influenzae and Haemophilus parainfluenzae with PathoTec strips and spot biochemical tests.

PathoTec strips and spot biochemical tests were evaluated for the ability to biotype Haemophilus influenzae and Haemophilus parainfluenzae. Indole, urease, and ornithine decarboxylase reactions were tested. The results of PathoTec strips compared favorably with those conventional methods; the percent agreements were as follows: indole, 100; urease, 99.5; and ornithine, 95.5. Spot tests were sim...

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Comparison of two commercial kits for identifying and biotyping Haemophilus parainfluenzae.

The Minitek system and the more recently introduced Micro Scan HNID panels for the identification and biotyping of 98 V dependent Haemophilus isolates were compared. Identical results were obtained for 77 isolates. The discrepancy in the results of ornithine and urease was accounted for mainly by the mismatching of the identification by the two kits. When 13 isolates of H parainfluenzae with mi...

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Methylase activities from Haemophilus influenzae that protect Haemophilus parainfluenzae transforming deoxyribonucleic acid from inactivation by Haemophilus influenzae endonuclease R.

Specific methylases that have the properties of deoxyribonucleic acid (DNA) modification enzymes have been isolated from Haemophilus influenzae strain Rd. Two activities ((Methylase IIa and methylase III) were found to protect transforming DNA of H. parainfluenzae from the action of H. influenzae restriction enzymes. To determine the specificty of the protection, a procedure based on biological...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2007

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.01663-07